Background: There are still a large variety of microorganisms among aquatic animals which have not been\nexplored for their pharmacological potential. Hence, present study was aimed to isolate and characterize a potent\nlactic acid bacterium from fresh water fish sample Zacco koreanus, and to confirm its pharmacological potential.\nMethods: Isolation of lactic acid bacteria (LAB) from fresh water fish samples was done using serial dilution\nmethod. Biochemical identification and molecular characterization of selected LAB isolate 1I1, based on its potent\nantimicrobial efficacy, was accomplished using API kit and 16S rRNA gene sequencing analysis. Further, 1I1 was\nassessed for Ã?±-glucosidase and tyrosinase inhibitory potential as well as antiviral efficacy against highly pathogenic\nhuman influenza virus H1N1 using MDCK cell line in terms of its pharmacological potential.\nResults: Here, we first time report isolation as well as biochemical and molecular characterization of a lactic acid\nbacterium Lactobacillus sakei 1I1 isolated from the intestine of a fresh water fish Z. koreanus. As a result, L. sakei\n1I1 exhibited potent antimicrobial effect in vitro, and diameter of zones of inhibition of 1I1 against the tested\npathogens was found in the range of 13.32 Ã?± 0.51 to 23.16 Ã?± 0.32 mm. Also L. sakei 1I1 at 100 mg/ml exhibited\nsignificant (p < 0.05) Ã?±ââ?¬â??glucosidase and tyrosinase inhibitory activities by 60.69 and 72.59 %, in terms of\nits anti-diabetic and anti-melanogenic potential, respectively. Moreover, L. sakei 1I1 displayed profound\nanti-cytopathic effect on MDCK cell line when treated with its ethanol extract (100 mg/ml), confirming its\npotent anti-viral efficacy against H1N1 influenza virus.\nConclusions: These findings reinforce the suggestions that L. sakei 1I1 isolated from the intestine of fresh water fish Z.\nkoreanus might be a candidate of choice for using in pharmacological preparations as an effective drug.
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